Description
SH3BGRL, also known as SH3 domain-binding glutamic acid-rich-like protein, is a member of the human SH3BGR family. SH3BGRL gene located to chromosome Xq13.3 encodes for a small protein of 114 amino acids, which is apparently widely expressed in many tissues including liver and blood. SH3BGRL protein features a prolinerich sequence (PLPPQIF), which contains both the SH3 binding (PXXP)3 and the Homer EVH1 binding (PPXXF)4 motif. This proline-rich region is highly conserved in human SH3BGR family and was expected to be functionally important. Recombinant human SH3BGRL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography